| School |
Last Year |
Notes |
| Adelphi University | 1953 | |
| Alabama Presbyterian College | 1917 | closed in 1918 |
| University of Alaska Fairbanks | 1952 | |
| Allen University | 2005 | |
| Alliance College | 1949 | closed in 1987 |
| Aquinas College | 1951 | |
| Arnold College | 1953 | merged with University of Bridgeport |
| Athens State University | 1949 | |
| Atlantic University | 1930 | |
| Atlantic Christian College | 1950 [3] | now Barton College |
| Aurora University | 1952 | |
| University of Baltimore | 1935 | |
| Baptist Christian | 1982 | |
| Bard College | 1924 | |
| Battle Creek College | 1926 | now Andrews University |
| Beaver College | 1904 | now Arcadia University |
| Bishop College | 1986 | closed and absorbed by Paul Quinn College |
| Blackburn College | 2008 | |
| Bluefield State College | 1980 | |
| Boston State College | 1974 | absorbed into UMass Boston |
| Bradley University | 1970 | |
| Brandeis University | 1959 [4] | |
| University of Bridgeport | 1975 | |
| Broaddus College | 1921 | merged into Alderson Broaddus University, Alderson Broaddus reinstated football in Division II in 2013 [5] |
| Brooklyn College | 1990 | |
| California Baptist University | 1955 | |
| California Institute of Technology | 1993 | |
| California State Polytechnic University, Pomona | 1985 | |
| California State University, Chico | 1996 | |
| California State University, Hayward | 1993 | now California State University, East Bay |
| California State University, Los Angeles | 1977 | |
| University of California, San Diego | 1968 [7] | |
| Cameron University | 1992 | |
| Canterbury College (Indiana) | 1950 | |
| Cedarville University | 1953 | |
| Champlain College | 1953 | |
| Christian Brothers University | 1922 | |
| Claflin University | 1965 | |
| Clarke Memorial College | 1933 | |
| Clarkson University | 1952 | |
| Colorado College | 2008 [8] | |
| Concordia College Alabama | 2016 | |
| Cooper Union | 1933 | |
| Daniel Baker College | 1951 | |
| Davis & Elkins College | 1953 1962 | |
| Detroit Institute of Technology | 1951 | |
| Dillard University | 1965 | |
| University of the District of Columbia | 1974 1990 | |
| Drury University | 1932 | |
| College of Emporia | 1972 [9] | |
| Erskine College | 1952 | |
| Fairleigh Dickinson University (Teaneck campus) | | Fairleigh Dickinson's other campus, Florham, currently has a football program in Division III |
| Federal City | 1974 | merged with D.C. Teachers College and Washington Technical Institute to form the University of the District of Columbia |
| Fisk University | 1983 | |
| Florida N&I | 1958 | now Florida Memorial University |
| Florida Southern College | 1935 | |
| Fort Lauderdale College | 1981 | now a part of the Everest University system |
| Frederick College | 1967 [10] | |
| Georgia Southwestern State University | 2003 | |
| Georgia State College | 1932 | now Abraham Baldwin Agricultural College |
| Haskell Indian Nations University | 2014 | |
| Haverford College | 1971 | |
| Hiram Scott College | 1970 | |
| Huntington College | 1949 | |
| Jarvis Christian College | 1966 | |
| Keene State College | 1929 | |
| King College | 2003 | |
| Knoxville College | 1997 | |
| Lake Superior State University | 1950 | |
| Lambuth University | 2010 | closed in 2011 |
| Lees–McRae College | 1993 | |
| Leland College | 1959 | closed in 1960 |
| LeMoyne–Owen College | 1950 | |
| Lewis University | 1956 | |
| Lincoln Memorial University | 1931 | |
| Louisville Municipal | 1951 | now Simmons College of Kentucky |
| Loyola University New Orleans | 1939 | |
| Lubbock Christian University | 1982 | |
| Mansfield University of Pennsylvania | 2006 | Mansfield began play in sprint football in 2008 |
| University of Massachusetts Boston | 2000 | |
| University of Massachusetts Lowell | 2002 | |
| Menlo College | 2015 | |
| Milligan College | 1950 | |
| Milton College | 1982 | closed in 1982 |
| Mississippi Industrial College | 1964 | closed in 1982 |
| Montana State University - Billings | 1978 | |
| Morris College | 1964 | |
| Morris Brown College | 2003 | |
| Nebraska Central College | 1951 | |
| University of Nebraska Omaha | 2010 | last played football as a Division II school, dropped the sport as part of its move to Division I |
| New Bedford Textile | 1950 | now New Bedford Institute of Technology |
| New England College | 1973 | |
| New Jersey City University | 2002 | |
| New York University | 1952 | |
| Polytechnic University of New York | 1902 | Polytechnic is now the engineering school of New York University, and known informally as "NYU Poly"; the NYU Poly athletic program merged into that of NYU effective with the 2014–15 school year |
| State University of New York at Farmingdale | 1937 | |
| State University of New York at Fredonia | 1916 | |
| State University of New York at Geneseo | 1913 | |
| State University of New York at New Paltz | 1936 | |
| State University of New York at Oswego | 1976 | |
| State University of New York at Plattsburgh | 1978 | |
| State University of New York at Potsdam | 1903 | |
| New York Institute of Technology | 1983 | |
| Norman College, GA | 1940 | closed in 1971 |
| University of North Dakota-Ellendale | 1971 | |
| North Georgia College | 1931 | now University of North Georgia |
| Northeastern Illinois University | 1988 | |
| Northern Idaho College of Education | 1950 | now Lewis–Clark State College |
| Northland College (Wisconsin) | 1975 | |
| Northwood University, TX | 1971 | |
| Oakland University | 1957 | |
| Oglethorpe University | 1940 | |
| Oklahoma City University | 1950 | |
| Oregon Institute of Technology | 1992 | |
| College of the Ozarks | 1967 [11] | now University of the Ozarks |
| Panzer College | 1950 | merged with Montclair State University in 1958 |
| Parsons College | 1970 | closed in 1973 |
| Paul Quinn College | 2006 | |
| Philadelphia University | 1900 | |
| Philander Smith College | 1964 | |
| Phillips University | 1933 | closed in 1998 |
| Piedmont College | 1940 | |
| The Principia | 2008 | |
| Quantico Marines | 1972 | |
| Queens College | 1974 | |
| Ramapo College | 1992 | |
| Reed College | 1952 | |
| Regis University | 1942 | |
| University of Rio Grande | 1949 | |
| Roanoke College | 1942 | |
| Rochester Institute of Technology | 1977 | |
| Roger Williams University | 1987 | |
| Rollins College | 1950 | |
| Rust College | 1964 | |
| Saint Leo University | 1980 | |
| Saint Martin's University | 1951 | |
| St. Mary of the Plains College | 1991 | |
| Saint Mary's University of Minnesota | 1954 | |
| St. Mary's University, Texas | 1941 | |
| Saint Michael's College, NM | 1950 | now Santa Fe University of Art and Design |
| Saint Michael's College, VT | 1953 | |
| Saint Paul's College | 2010 | closed in 2013 |
| Samuel Huston college | 1951 | now Huston–Tillotson University |
| San Francisco State University | 1994 | |
| The University of Scranton | 1960 | |
| Selma University | 1940 | |
| Shaw College at Detroit | 1951 | |
| Shurtleff College | 1950 | |
| Si Tanka University | 2004 | |
| Sonoma State University | 1997 | |
| South Carolina Trade | 1966 | now Midlands Technical College |
| University of South Dakota - Springfield | 1982 | |
| South Georgia College | 1940 | |
| Southern California College | 1961 | now Vanguard University of Southern California |
| Southern Idaho College of Education | 1950 | closed in 1951 |
| Spring Hill College | 1941 [12] | |
| College of Steubenville | 1949 | now Franciscan University of Steubenville |
| Stevens Institute of Technology | 1924 | |
| Sue Bennett College | 1997 | closed and absorbed by Union College (KY) |
| Swarthmore College | 2000 | |
| Talladega College | 1940 | |
| University of Tampa | 1974 | |
| Tarkio College | 1990 | closed in 1992 |
| Tennessee Wesleyan College | 1993 | |
| Texas Wesleyan University | 1941 | will reinstate football for the 2017 season |
| Tillotson College | 1950 | now Huston–Tillotson University |
| Tougaloo College | 1960 | |
| Transylvania University | 1941 | |
| U.S. International University | 1980 | now Alliant International University |
| Union University | 1952 | |
| Upsala College | 1993 | |
| Washington College | 1950 | |
| Webb Institute | 1931 | |
| West Virginia University Institute of Technology | 2011 | |
| Western Reserve University | 1967 | merged with Case Institute of Technology to form Case Western Reserve University |
| Western Washington University | 2008 | |
| Westmar University | 1997 | |
| Westminster College (Utah) | 1978 | |
| Whitman College | 1976 | |
| Wiley College | 1968 | |
| William Carey University | 1955 | |
| Wisconsin Extension | 1955 | merged with Wisconsin State College of Milwaukee to form today's University of Wisconsin–Milwaukee |
| University of Wisconsin–Superior | 1992 | |
| Xavier University of Louisiana | 1959 | |
| Yankton College | 1983 | |
| York College (Nebraska) | 1953 | |