Miltiradiene synthase

Miltiradiene synthase (EC 4.2.3.131, SmMDS, SmiKSL) is an enzyme with systematic name (+)-copaly-diphosphate diphosphate-lyase (cyclizing, miltiradiene-forming).^[1]^[2] This enzyme catalyses the following chemical reaction

(+)-copalyl diphosphate

\rightleftharpoons

miltiradiene + diphosphate

This enzyme is isolated from the plant Selaginella moellendorffii.

References

↑ Gao, W., Hillwig, M.L., Huang, L., Cui, G., Wang, X., Kong, J., Yang, B. and Peters, R.J. (2009). "A functional genomics approach to tanshinone biosynthesis provides stereochemical insights". Org. Lett. 11: 5170–5173. doi:10.1021/ol902051v. PMID 19905026.
↑ Sugai, Y., Ueno, Y., Hayashi, K., Oogami, S., Toyomasu, T., Matsumoto, S., Natsume, M., Nozaki, H. and Kawaide, H. (2011). "Enzymatic ¹³C labeling and multidimensional NMR analysis of miltiradiene synthesized by bifunctional diterpene cyclase in Selaginella moellendorffii". J. Biol. Chem. 286: 42840–42847. doi:10.1074/jbc.m111.302703. PMID 22027823.

Miltiradiene synthase at the US National Library of Medicine Medical Subject Headings (MeSH)

Carbon-oxygen lyases (EC 4.2) (primarily dehydratases)

4.2.1: Hydro-Lyases	Carbonic anhydrase Fumarase Aconitase Enolase Alpha Enolase 2 Enoyl-CoA hydratase/3-Hydroxyacyl ACP dehydrase Methylglutaconyl-CoA hydratase Tryptophan synthase Cystathionine beta synthase Porphobilinogen synthase 3-Isopropylmalate dehydratase Urocanase Uroporphyrinogen III synthase Nitrile hydratase

4.2.2: Acting on polysaccharides	Hyaluronate lyase

4.2.3: Acting on phosphates	Threonine synthase

4.2.99: Other	Carboxymethyloxysuccinate lyase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list)

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